Characterisation of uropathogenic E.coli by detecting the virulence factors and its drug resistance pattern in a tertiary care hospital in India

Urinary tract infections (UTIs) are among the most prevalent nosocomial and community-acquired bacterial diseases in humans, with E.coli being typical pathogen isolated. To detect prevalence of virulence factors like haemolysin, haemagglutination human erythrocytes its effect D-mannose, cell surface hydrophobicity, antibiotic sensitivity pattern ESBL production urinary isolates obtained from clinical samples. We included a midstream urine sample for study. Virulence hemagglutination salt aggregation were detected as per standard protocols. Antibiotic testing was performed by Kirby Bauer disc diffusion method. Extended-spectrum beta-lactamase (ESBL) seen combined method on Muller Hinton agar CLSI guidelines. A total 103 tested, them, 24(23.30%) produced 65(63.10%) 38(36.89%) had properties. Most resistant to beta-lactam antibiotics but showed high towards chloramphenicol, meropenem, amikacin, imipenem nitrofurantoin. Around 48% them producers. The common associated UTI P-fimbriae (MRHA), haemolysin production, hydrophobicity type-1 fimbriae. Because emerging drug resistance UPEC, therapy should be advocated far possible after obtaining culture results determine exact aetiology susceptibility patterns. nitrofurantoin is very high, suggesting that recycling will help clinicians treat UPEC.